Phenolic acid derivatives from Bauhinia glauca subsp. pernervosa / 药学学报

To study the chemical constituents of Bauhinia glauca subsp. pernervosa, eleven phenolic acids were isolated from a 95% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, MCI, Sephadex LH-20, and semi-preparative HPLC. By spectroscopic techniques including 1H NMR, 13C NMR, 2D NMR, and HR-ESI-MS, these compounds were identified as isopropyl O-beta-(6'-O-galloyl)-glucopyranoside (1), ethyl O-beta-(6'-O-galloyl)-glucopyranoside (2), 3, 4, 5-trimethoxyphenyl-(6'-O-galloyl)-O-beta-D-glucopyranoside (3), 3, 4, 5-trimethoxyphenyl-beta-D-glucopyranoside (4), gallic acid (5), methyl gallate (6), ethyl gallate (7), protocatechuic acid (8), 3, 5-dimethoxy-4-hydroxybenzoic acid (9), erigeside C (10) and glucosyringic acid (11). Among them, compound 1 is a new polyhydroxyl compound; compounds 2, 10, and 11 were isolated from the genus Bauhinia for the first time, and the other compounds were isolated from the plant for the first time. Compounds 6 and 8 showed significant protein tyrosine phosphatase1B (PTP1B) inhibitory activity in vitro with the IC50 values of 72.3 and 54.1 micromol x L(-1), respectively.

Preliminary investigation of orthodontic treatment in compliance with dentofacial development in patients with skeletal Class III malocclusion and open bite / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the biologic principle of orthodontic treatment in patients with skeletal Class III malocclusion and open bite.</p><p><b>METHODS</b>Eleven pre-adolescent patients with severe skeletal Class III malocclusion and open bite (age range 7 - 9 years old, mean age 8.3 +/- 0.8) were included. All patients were surgical cases but the patients rejected surgery. The treatment methods used were face mask, rapid maxillary expansion occlusal splint and fixed appliance. Lateral cephalometric films were taken before and after treatment. Cephalometric analysis was performed.</p><p><b>RESULTS</b>After the treatment SNA changed from (79.0 +/- 1.2) degrees to (81.9 +/- 0.8) degrees (P < 0.01). And the inclination of lower incisors was decreased from (25.6 +/- 2.1) degrees to (20.1 +/- 1.4) degrees when measured to the NB line (P < 0.01). The direction of the facial growth was maintained.</p><p><b>CONCLUSIONS</b>Good orthodontic results could be achieved in patients with skeletal Class III malocclusion and open bite.</p>

Treatment of maxillary protrusion in adult with self-tapping micro-screw implants / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the position change of upper molars and incisors in order to evaluate the stability of posterior anchorage with the application of micro-screw implant.</p><p><b>METHODS</b>Eight adult patients with severe maxillary protrusion were included. Upper first premolars were extracted and upper posterior anchorage was reinforced with micro-screw implant in all patients. Cephalometric and cast analyses were carried out to record the position change of molar and micro-screw.</p><p><b>RESULTS</b>During treatment the micro-screw implants kept stable in sagittal and vertical plane. Neither the mesial-distal movement nor the tipping of the upper molars during the treatment was statistically significant (P > 0.05). The edge of upper incisors was retracted by 6.86 mm and the tipping was reduced by 18.03 degrees . The center of resistance was intruded by 3.28 mm on average. Significant change was observed (P < 0.01).</p><p><b>CONCLUSIONS</b>Micro-screw implant could provide good anchorage control in the orthodontic treatment.</p>

Study on biligustilides from Angelica sinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Angelica sinensis.</p><p><b>METHOD</b>The constituents were separated by chromatographic methods, and their structures were identified on the basis of spectroscopic analysis.</p><p><b>RESULT</b>Eight compounds were isolated and identified as levistolide A (1), senkyunolide O (2), (3Z, 3Z')-6.8', 7.3'-diligustilide (3), tokinolide B (4), isotokinolide B (5), (3'Z)-(3R, 8S, 3a'R, 6'S)-3, 3a': 8, 6'-biligustilide (6), E, E'-3. 3', 8. 8'-diligustilide (7) and E, E'-3. 3', 8. 8'-isodiligustilide (8), which are all diligustilides.</p><p><b>CONCLUSION</b>Compound 7 was obtained from the plant for the first time; compounds 6 and 8 are new compounds.</p>

N01WA-735E, a human leukocyte elastase inhibitor from metabolites of microorganisms / 生物工程学报

Human leukocyte elastase is an important selection target of inflammation and cancer. In this paper, a high throughput screening model was established for screening human leukocyte elastase inhibitors from thousands of strains of actinomycetes. As a result, a strain, N01WA-735 with potent suppression activity was isolated. Firstly, the strain N01WA-735 was identified as Streptomyces according to morphology and biochemical analysis. The Streptomyces N01WA-735 was processed by solvent extraction, silica column chromatography, Sephadex LH-20 column chromatography and crystallization to get a pure active compound named N01WA-735E. Its chemical structure was elucidated as the same as that of the compound named BE-52440A by physicochemical properties and spectral data of UV, MS, 1H-NMR and 13C-NMR respectively. The compound showed a strong inhibitory activity against human leukocyte elastase with IC50 of 3.02 micromol/L. The compound is reported as a human leukocyte elastase inhibitor for the first time.

Three-dimensional finite element analysis of distalization of lower cuspid with light-segmented archwire technique / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the mechanics of distalizating lower cuspid with the light-segmented archwire.</p><p><b>METHODS</b>An experiment, which imitated the loading of distalizating lower cuspid with the light-segmented archwire, was performed on the three-dimensional finite element method model of lower cuspid. The patterns of stress distribution of the root were analyzed.</p><p><b>RESULTS</b>Under the loading of the light-segmented archwire, the lower cuspid root presented an even pressing force distribution on the distal and lingual side and an even stretching force distribution on the mesial and buccal side.</p><p><b>CONCLUSIONS</b>The light-segmented archwire would lead to bodily movement of the cuspid.</p>

The megastigman glycosides from herb of Potentilla multifida / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Potentilla multifida.</p><p><b>METHOD</b>Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by spectral analysis.</p><p><b>RESULT</b>Four megastigmane glycosides were isolated from P. multifida and their structures were identified as citroside A (1), icariside B1 (2), (6S,7E,9R)-roseoside (3), (6S,7E,9R)-vomifoliol-9-O-beta-D-xylopyranosyl-(1-->6)-O-beta-D-glucopyranoside (4), respectively.</p><p><b>CONCLUSION</b>All compounds were obtained from the genus Potentilla for the first time.</p>

Analysis of distalization of lower canine by light-segmented archwire / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the practical effect of distalization of lower cuspiud by light-segmented archwire.</p><p><b>METHODS</b>17 cases were selected. In all cases, two lower first bicuspids were extracted and lower molars were designed as reinforced anchorage. In the first half year, lower cuspids were distalized with light-segmented archwire, and lower incisors were in the physiologic drift stage. Before the treatment, three months and six months after the treatment, cephalograms and study models were recorded respectively. The data were analyzed with SPSS 10.0.</p><p><b>RESULTS</b>The distance of mesiolization of the first lower molar was 1.14 mm in half a year. The distance of distalization of the lower cuspid was 5.02 mm in half a year. No significant difference was found in LM-MP and SN-MP angle. LI-NB angle was decreased by 9.57 degrees. Crowding of lower incisors was transferred from -2.55 mm to 1.08 mm.</p><p><b>CONCLUSION</b>During distalization of lower cuspid by light-segmented archwire, anchorage tooth was stable, lower cuspids were distalized effectively, and lower incisors drifted towards the ideal position automatically.</p>

Changes of cranio-facial hard tissue after orthodontic treatment in bimaxillary protrusive patients / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study is to investigate the changes of hard tissue profile in anterior-posterior and vertical direction in bimaxillary protrusion patients after orthodontic treatment.</p><p><b>METHODS</b>A total of 24 bimaxillary protrusion patients (male 8, female 16), aged from 11.2 to 29.0 (average 16.9 years old), were selected to be treated with standard edgewise technique consisted of 4 first premolars extraction. Cephalometrics were taken before and after treatment. The changes of hard tissue profile were studied using the computer-aid X-ray cephalometric analysis.</p><p><b>RESULTS</b>1. The length of the maxillary and the mandible increased significantly, but the anterior-posterior relationship of the maxillary and the mandible did not change significantly. 2. The anterior and posterior facial height increased significantly, but the ratio of anterior and posterior facial height and the angle of MP-FH which reflected the inclination of the mandible plane did not change significantly. 3. The height of the upper and lower first molar increased significantly along with the increase of the anterior and posterior facial height. 4. The height of the upper incisors increased significantly, but the height of the lower incisors decreased significantly.</p><p><b>CONCLUSION</b>The anchorage in anterior-posterior and vertical direction were controlled preferably, the patients did not manifest disadvantageous vertical growth trend.</p>

Isolation and identification of the ligustilide compounds from the root of Angelica sinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To find out substance basis of pharmacological activities of Angelica sinensis.</p><p><b>METHOD</b>Chromatographic methods were used to isolate the chemical components, and spectroscopic methods were used to identify their structures.</p><p><b>RESULT</b>Five compounds were isolated from the ethanol extract of the roots of Angelica sinensis. Their structures were identified as: (Z)-ligustilide, (Z)-6,7-epoxyligustilide, (Z)-6,7-cis-dihydroxyligustilide, (E)-6,7-cis-dihydroxy-ligustilide and 11-angeloylsenkyunolide F.</p><p><b>CONCLUSION</b>11-angeloylsenkyunolide F was obtained from Angelica sinensis for the first time.</p>

The effectiveness of EUS in detecting islet cell tumor / 中华消化内镜杂志

Objective To study on the effectiveness of endoscopic ultrasonography(EUS)in detec- ting insulinoma preoperatively.Methods Fifteen patients with clinical and biochemical signs of insulinoma were examined by EUS using a radial-scanning ultrasound endoscope and abdominal ultrasonography,CT, DSA prior to surgery.The outcome was evaluated on the basis of surgery and examination of the resected specimens.Results Fifteen patients with 16 lesions of insulinoma were identified by surgery and pathology. The aceuraey of diagnosis with EUS was 13/15(86.7%),and that with B-US,CT,DSA was 3/15(20%), 5/15(33.3%),9/14(64.3%)respectively.In the 14 lesions identified by EUS,10 lesions were depicted to be hypoechogenic,1 lesion was isoechogenic and 3 lesions were hyperechogenie.All 14 lesions were well demarcated and surrounded by normal pancreatic tissue.The minimum size of the lesion visualized by EUS was 0.5cm.Ten lesions were correctly detected by EUS with size of 0.5～2.0cm.EUS missed diagnosis in 2 lesions not for their small size.EUS falsely indicated a 10mm lesion from two lesions inside the head of pancreas.One lesion outside the pancreatic tail and one lesion in the pancreatic head were missed by EUS in another case.Conclusion EUS is superior in assessing the location of pancreatic insulinoma than other ima- ging methods such as B-US,CT,DSA.

Construction of retrovirus vector containing rat cardiac myosin α heavy chain cDNA / 第二军医大学学报

Objective： To clone rat cardiac myosin α he avy chain cDNA fragment encoding aa736-960 and construct its recombinant retrov irus vector(RV). Methods: The 681 bp target gene was amplified f rom heart tissue of young rats with RT-PCR, fusion gene of huIL-2/myosin was c onstructed by splicing with huIL-2 cDNA using ligation methods and its RV was constructed. RT-PCR and immunohistochemical assay were used to iden tify the expression of myosin protein in transfected cell. Results: The determination of nucleotide sequence showed that the nucleotide and ami no acid sequence of gene clone was the same as reported, its openin g reading frame was correct, the digesting result of pLNC-huIL-2-myosin was i dentical with the predicted. NIH3T3 cell was transfected with recombinant RV, and G418-resistant NIH3T3 cell was established.RT-PCR analysis indicated tha t mRNA of pLNC-huIL-2-myosin was present in cell transfected with RV. The im munohistochemical assay also showed that the myosin protein expression was highe r in the cell transfected with constructed RV. Conclusion: Rat cardiac myosin α heavy chain cDNA has been cloned and its RV has also been cons tructed and expressed in NIH3T3 successfully, it will contribute to research of prevention and treatment of heart immune injury by cardiac myosin gene transfer to induce specific tolerance.

Construction of retrovirus vector containing rat cardiac myosin α heavy chain cDNA / 第二军医大学学报

Objective： To clone rat cardiac myosin α he avy chain cDNA fragment encoding aa736-960 and construct its recombinant retrov irus vector(RV). Methods: The 681 bp target gene was amplified f rom heart tissue of young rats with RT-PCR, fusion gene of huIL-2/myosin was c onstructed by splicing with huIL-2 cDNA using ligation methods and its RV was constructed. RT-PCR and immunohistochemical assay were used to iden tify the expression of myosin protein in transfected cell. Results: The determination of nucleotide sequence showed that the nucleotide and ami no acid sequence of gene clone was the same as reported, its openin g reading frame was correct, the digesting result of pLNC-huIL-2-myosin was i dentical with the predicted. NIH3T3 cell was transfected with recombinant RV, and G418-resistant NIH3T3 cell was established.RT-PCR analysis indicated tha t mRNA of pLNC-huIL-2-myosin was present in cell transfected with RV. The im munohistochemical assay also showed that the myosin protein expression was highe r in the cell transfected with constructed RV. Conclusion: Rat cardiac myosin α heavy chain cDNA has been cloned and its RV has also been cons tructed and expressed in NIH3T3 successfully, it will contribute to research of prevention and treatment of heart immune injury by cardiac myosin gene transfer to induce specific tolerance.